Diagnostic role of BIOMED-2 multiplex polymerase chain reaction for antigen receptor genes rearrangement in lymphoproliferative disorders / 中华血液学杂志
Chinese Journal of Hematology
; (12): 251-254, 2009.
Article
in Zh
| WPRIM
| ID: wpr-314494
Responsible library:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To establish a sensitive and effective method for detection of immunoglobulin and T-cell receptor (Ig/TCR) gene rearrangement,and to explore its role in diagnosis and differential diagnosis of lymphoproliferative disorders.</p><p><b>METHODS</b>Fifty-eight lymphoid tissue samples from 54 patients with lymphoproliferations were evaluated by the novel BIOMED-2 multiplex polymerase chain reaction (PCR) for antigen receptor genes rearrangement.</p><p><b>RESULTS</b>Multiplex PCR demonstrated monoclonal Ig/TCR gene rearrangements in 22 of 25 (88.0%) B-cell malignancies and 8 of 15 (53.3%) T-cell malignancies. Among 17 benign lymphoproliferations confirmed histopathologically, polyclonal rearrangements were detected in 14 cases (82.4%). In total, the clonality analysis and the final clinico-histopathological diagnosis were concordant in 77.2%. Combination detection of Iglambda and TCR delta gene rearrangements did not increase the detection rate of monoclonal rearrangement of Ig/TCR, but might help to the detection of Iglambda+ or TCR delta+ lymphomas.</p><p><b>CONCLUSION</b>The novel BIOMED-2 multiplex PCR strategy is a rapid, reliable and sensitive approach to detecting clonality in suspected lymphoproliferations, especially in atypical cases.</p>
Full text:
1
Database:
WPRIM
Main subject:
Gene Rearrangement, B-Lymphocyte, Light Chain
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Gene Rearrangement, T-Lymphocyte
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Polymerase Chain Reaction
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Sensitivity and Specificity
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Diagnosis
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Genetics
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Lymphoproliferative Disorders
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Methods
Type of study:
Diagnostic_studies
Limits:
Female
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Humans
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Male
Language:
Zh
Journal:
Chinese Journal of Hematology
Year:
2009
Document type:
Article
Country of publication:
CHINA
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CN
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REPUBLIC OF CHINA